Browsing by Author "Rose R., Christine"

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    Petersilie et al. 2024b - Figure 3
    (STAR Protocols, 2024) Petersilie, Laura; Kafitz, Karl W.; Neu, Louis A.; Heiduschka, Sonja; Le, Stephanie; Prigione, Alessandro; Rose R., Christine
    Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.