Petersilie, LauraKafitz, Karl W.Neu, Louis A.Heiduschka, SonjaLe, StephaniePrigione, AlessandroRose, Christine R.2024-06-052024-06-052024https://researchdata.hhu.de/handle/entry/16310.25838/d5p-68Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.enNATURAL SCIENCES::Biology::Cell and molecular biology::NeurobiologyArticle