Institute of Neurobiology

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The Institute of Neurobiology at the HHU is devoted to the elucidation and study of intracellular ion dynamics in the vertebrate brain under physiological as well as pathophysiological conditions.

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Browsing Institute of Neurobiology by Subject "NATURAL SCIENCES::Biology::Cell and molecular biology::Neurobiology"

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    Petersilie et al. 2024b - Figure 1
    (STAR Protocols, 2024) Petersilie, Laura; Kafitz, Karl W.; Neu, Louis A.; Heiduschka, Sonja; Le, Stephanie; Prigione, Alessandro; Rose, Christine R.
    Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.
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    Petersilie et al. 2024b - Figure 2
    (STAR Protocols, 2024) Petersilie, Laura; Kafitz, Karl W.; Neu, Louis A.; Heiduschka, Sonja; Le, Stephanie; Prigione, Alessandro; Rose, Christine R.
    Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.
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    Petersilie et al. 2024b - Figure 2
    (STAR Protocols, 2024) Petersilie, Laura; Kafitz, Karl W.; Neu, Louis A.; Heiduschka, Sonja; Le, Stephanie; Prigione, Alessandro; Rose, Christine R.
    Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.
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    Petersilie et al. 2024b - Figure 3
    (STAR Protocols, 2024) Petersilie, Laura; Kafitz, Karl W.; Neu, Louis A.; Heiduschka, Sonja; Le, Stephanie; Prigione, Alessandro; Rose R., Christine
    Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.
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    Petersilie et al. 2024b - Figure 4
    (STAR Protocols, 2024) Petersilie, Laura; Kafitz, Karl W.; Neu, Louis A.; Heiduschka, Sonja; Le, Stephanie; Prigione, Alessandro; Rose, Christine R.
    Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.
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    Petersilie et al. 2024b - Figure 5
    (STAR Protocols, 2024) Petersilie, Laura; Kafitz, Karl W.; Neu, Louis A.; Heiduschka, Sonja; Le, Stephanie; Prigione, Alessandro; Rose, Christine R.
    Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.