Petersilie et al. 2024b - Figure 4
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Date
2024
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Publisher
STAR Protocols
Abstract
Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.
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NATURAL SCIENCES::Biology::Cell and molecular biology::Neurobiology