Petersilie et al. 2024b - Figure 4

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dc.contributor.authorPetersilie, Laura
dc.contributor.authorKafitz, Karl W.
dc.contributor.authorNeu, Louis A.
dc.contributor.authorHeiduschka, Sonja
dc.contributor.authorLe, Stephanie
dc.contributor.authorPrigione, Alessandro
dc.contributor.authorRose, Christine R.
dc.date.accessioned2024-06-10T13:10:18Z
dc.date.available2024-06-10T13:10:18Z
dc.date.issued2024
dc.description.abstractThree-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.
dc.identifier.urihttps://researchdata.hhu.de/handle/entry/174
dc.language.isoen
dc.publisherSTAR Protocols
dc.subjectNATURAL SCIENCES::Biology::Cell and molecular biology::Neurobiology
dc.titlePetersilie et al. 2024b - Figure 4
dc.title.alternativeProtocol for the generation of cultured cortical brain organoid slices (cBOS)
dc.typeArticle

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