The Medical Faculty

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The Faculty of Medicine evolved out of the former Academy of Applied Medicine, which was established in 1907. It soon achieved international recognition when leading practitioners pioneeringly integrated basic research and clinical practice.

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    Loss of Bmal1 impairs the glutamatergic light input to the SCN in mice
    (NA, 2024-12-03) Korkmaz, Hüseyin; Anstötz, Max; Wellinghof, Tim; Fazari, Benedetta; Hallenberger, Angelika; Bergmann, Ann Kathrin; Niggetiedt, Elena; Güven, Delal; Tundo-Lavalle, Federica; Purath, Fathima Faiba A.; Bochinsky, Kevin; Gremer, Lothar; Willbold, Dieter; von Gall, Charlotte; Ali, Amira A. H.
    Introduction: Glutamate represents the dominant neurotransmitter that conveys the light information to the brain, including the suprachiasmatic nucleus (SCN), the central pacemaker for the circadian system. The neuronal and astrocytic glutamate transporters are crucial for maintaining efficient glutamatergic signaling. In the SCN, glutamatergic nerve terminals from the retina terminate on vasoactive intestinal polypeptide (VIP) neurons, which are essential for circadian functions. Up-to-date, little is known about the role of the core circadian clock gene, Bmal1, in glutamatergic neurotransmission of light signal to various brain regions. Methods: The aim of this study was to further elucidate the role of Bmal1 in glutamatergic neurotransmission from the retina to the SCN. We therefore examined the spontaneous rhythmic locomotor activity, neuronal and glial glutamate transporters, as well as the ultrastructure of the synapse between the retinal ganglion cells (RGCs) and the SCN in adult male Bmal1-/- mice. Results: We found that the deletion of Bmal1 affects the light-mediated behavior in mice, decreases the retinal thickness and affects the vesicular glutamate transporters (vGLUT1,2) in the retina. Within the SCN, the immunoreaction of vGLUT1,2, glial glutamate transporters (GLAST) and VIP was decreased while the glutamate concentration was elevated. At the ultrastructure level, the presynaptic terminals were enlarged and the distance between the synaptic vesicles and the synaptic cleft was increased, indicative of a decrease in the readily releasable pool at the excitatory synapses in Bmal1-/-. Conclusion: Our data suggests that Bmal1 deletion affects the glutamate transmission at the tripartite synapse between the ipRGCs and the VIP neurons in the SCN and affects the behavioral responses to light.
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    Reducing sources of variance in experimental procedures in in vitro research
    (N/A, 2021-08-30) Fischer, Igor; Martinez Dominguez, Maria Victoria; Hänggi, Daniel; Kahlert, Ulf Dietrich
    In this project, we statistically evaluated repetitive data of a very basic, in academic and industry lab most widely applied lab procedure, namely quantifying number of viable cells, to appreciate the impact of different parameters and instrumentations that may apply during this procedure as sources of variance. Moreover, by comparing the variations of data acquired under two different procedures featuring improved stringency of protocol adherence, our project attempts to propose guidelines how to reduce variations.
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    Subacute thyroiditis – Is it really linked to viral infection? - Supplemental material
    (The authors, 2025-01-22) Hans Martin Orth, Alexander Killer, Smaranda Gliga, Michael Böhm, Torsten Feldt, Björn-Erik O. Jensen, Tom Luedde, Rolf Kaiser, Martin Pirkl
    This document includes additional figures for the study "Subacute thyroiditis – Is it really linked to viral infection?"