Sequence alignments of alpha and beta spectrin

Abstract

*** Multiple sequence alignment of erythrocytic α-spectrin sequences from 58 different species Sequences of erythrocytic α-spectrin were collected from the UniProtKB database at uniprot.org. 58 sequences with “erythrocytic α-spectrin” annotation were selected with an additional criterium being a comparable sequence length to human erythrocytic α-spectrin (2,419 residues). Multiple sequence alignment was performed with Clustal Omega (webserver version accessed at https://www.ebi.ac.uk/Tools/msa/clustalo/ on 04.30.2020) with default parameters. The result is given in the file with the ending .clustal_num. In addition, the sequence alignment is displayed as a .tif file, with colored residues indicating high identiy according to the ‘identity’ coloring option of Mview; colors vary according to physicochemical properties as specified in the MView documentation (Madeira, Park et al. 2019).

*** Multiple sequence alignment of erythrocytic β-spectrin sequences from 11 different species Sequences of erythrocytic β-spectrin were collected from the UniProtKB database at uniprot.org. 11 sequences with “erythrocytic β -spectrin” annotation were selected with an additional criterium being a comparable sequence length to human erythrocytic β-spectrin (2,137 residues). Multiple sequence alignment was performed with Clustal Omega (webserver version accessed at https://www.ebi.ac.uk/Tools/msa/clustalo/ on 04.30.2020) with default parameters. The result is given in the file with the ending .clustal_num. In addition, the sequence alignment is displayed as a .tif file, with colored residues indicating high identiy according to the ‘identity’ coloring option of Mview; colors vary according to physicochemical properties as specified in the MView documentation (Madeira, Park et al. 2019).

*** Madeira, F., Park, Y. M., Lee, J., Buso, N., Gur, T., Madhusoodanan, N., Basutkar, P., Tivey, A. R. N., Potter, S. C., Finn, R. D., and Lopez, R. (2019) The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 47, W636-W641